RT Journal Article
SR Electronic
T1 Fluorescent tracking identifies key migratory dendritic cells in the lymph node after radiotherapy
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202101337
DO 10.26508/lsa.202101337
VO 5
IS 9
A1 Tiffany C Blair
A1 Shelly Bambina
A1 Gwen F Kramer
A1 Alexa K Dowdell
A1 Alejandro F Alice
A1 Jason R Baird
A1 Amanda W Lund
A1 Brian D Piening
A1 Marka R Crittenden
A1 Michael J Gough
YR 2022
UL https://www.life-science-alliance.org/content/5/9/e202101337.abstract
AB Radiation therapy generates extensive cancer cell death capable of promoting tumor-specific immunity. Within the tumor, conventional dendritic cells (cDCs) are known to carry tumor-associated antigens to the draining lymph node (TdLN) where they initiate T-cell priming. How radiation influences cDC migration is poorly understood. Here, we show that immunological efficacy of radiation therapy is dependent on cDC migration in radioimmunogenic tumors. Using photoconvertible mice, we demonstrate that radiation impairs cDC migration to the TdLN in poorly radioimmunogenic tumors. Comparative transcriptional analysis revealed that cDCs in radioimmunogenic tumors express genes associated with activation of endogenous adjuvant signaling pathways when compared with poorly radioimmunogenic tumors. Moreover, an exogenous adjuvant combined with radiation increased the number of migrating cDCs in these poorly radioimmunogenic tumors. Taken together, our data demonstrate that cDC migration play a critical role in the response to radiation therapy.