PT  - JOURNAL ARTICLE
AU  - Tiffany C Blair
AU  - Shelly Bambina
AU  - Gwen F Kramer
AU  - Alexa K Dowdell
AU  - Alejandro F Alice
AU  - Jason R Baird
AU  - Amanda W Lund
AU  - Brian D Piening
AU  - Marka R Crittenden
AU  - Michael J Gough
TI  - Fluorescent tracking identifies key migratory dendritic cells in the lymph node after radiotherapy
AID  - 10.26508/lsa.202101337
DP  - 2022 Sep 01
TA  - Life Science Alliance
PG  - e202101337
VI  - 5
IP  - 9
4099  - https://www.life-science-alliance.org/content/5/9/e202101337.short
4100  - https://www.life-science-alliance.org/content/5/9/e202101337.full
SO  - Life Sci. Alliance2022 Sep 01; 5
AB  - Radiation therapy generates extensive cancer cell death capable of promoting tumor-specific immunity. Within the tumor, conventional dendritic cells (cDCs) are known to carry tumor-associated antigens to the draining lymph node (TdLN) where they initiate T-cell priming. How radiation influences cDC migration is poorly understood. Here, we show that immunological efficacy of radiation therapy is dependent on cDC migration in radioimmunogenic tumors. Using photoconvertible mice, we demonstrate that radiation impairs cDC migration to the TdLN in poorly radioimmunogenic tumors. Comparative transcriptional analysis revealed that cDCs in radioimmunogenic tumors express genes associated with activation of endogenous adjuvant signaling pathways when compared with poorly radioimmunogenic tumors. Moreover, an exogenous adjuvant combined with radiation increased the number of migrating cDCs in these poorly radioimmunogenic tumors. Taken together, our data demonstrate that cDC migration play a critical role in the response to radiation therapy.