@article {Nalbandiane202101312, author = {Minas Nalbandian and Mingming Zhao and Hiroki Kato and Tatsuya Jonouchi and May Nakajima-Koyama and Takuya Yamamoto and Hidetoshi Sakurai}, title = {Single-cell RNA-seq reveals heterogeneity in hiPSC-derived muscle progenitors and E2F family as a key regulator of proliferation}, volume = {5}, number = {8}, elocation-id = {e202101312}, year = {2022}, doi = {10.26508/lsa.202101312}, publisher = {Life Science Alliance}, abstract = {Human pluripotent stem cell-derived muscle progenitor cells (hiPSC-MuPCs) resemble fetal-stage muscle progenitor cells and possess in vivo regeneration capacity. However, the heterogeneity of hiPSC-MuPCs is unknown, which could impact the regenerative potential of these cells. Here, we established an hiPSC-MuPC atlas by performing single-cell RNA sequencing of hiPSC-MuPC cultures. Bioinformatic analysis revealed four cell clusters for hiPSC-MuPCs: myocytes, committed, cycling, and noncycling progenitors. Using FGFR4 as a marker for noncycling progenitors and cycling cells and CD36 as a marker for committed and myocyte cells, we found that FGFR4+ cells possess a higher regenerative capacity than CD36+ cells. We also identified the family of E2F transcription factors are key regulators of hiPSC-MuPC proliferation. Our study provides insights on the purification of hiPSC-MuPCs with higher regenerative potential and increases the understanding of the transcriptional regulation of hiPSC-MuPCs.}, URL = {https://www.life-science-alliance.org/content/5/8/e202101312}, eprint = {https://www.life-science-alliance.org/content/5/8/e202101312.full.pdf}, journal = {Life Science Alliance} }