RT Journal Article
SR Electronic
T1 Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencing
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202101294
DO 10.26508/lsa.202101294
VO 5
IS 8
A1 Chu Cheng
A1 Pengfeng Xiao
YR 2022
UL https://www.life-science-alliance.org/content/5/8/e202101294.abstract
AB Next-generation sequencing (NGS) promises to revolutionize precision medicine, but the existing sequencing technologies are limited in accuracy. To overcome this limitation, we propose the correctable decoding sequencing strategy, which is a duplex sequencing protocol with conservative theoretical error rates of 0.0009%. This rate is lower than that for Sanger sequencing. Here, we simulate the sequencing reactions by the self-developed software, and find that this approach has great potential in NGS in terms of sequence decoding, reassembly, error correction, and sequencing accuracy. Besides, this approach can be compatible with most SBS-based sequencing platforms, and also has the ability to compensate for some of the shortcomings of NGS platforms, thereby broadening its application for researchers. Hopefully, it can provide a powerful new protocol that can be used as an alternative to the existing NGS platforms, enabling accurate identification of rare mutations in a variety of applications in biology and medicine.