PT  - JOURNAL ARTICLE
AU  - Nikhila Krishnan
AU  - Maxx Swoger
AU  - Lindsay I Rathbun
AU  - Peter J Fioramonti
AU  - Judy Freshour
AU  - Michael Bates
AU  - Alison E Patteson
AU  - Heidi Hehnly
TI  - Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo
AID  - 10.26508/lsa.202201362
DP  - 2022 Jul 01
TA  - Life Science Alliance
PG  - e202201362
VI  - 5
IP  - 7
4099  - https://www.life-science-alliance.org/content/5/7/e202201362.short
4100  - https://www.life-science-alliance.org/content/5/7/e202201362.full
SO  - Life Sci. Alliance2022 Jul 01; 5
AB  - The last stage of cell division involves two daughter cells remaining interconnected by a cytokinetic bridge that is cleaved during abscission. Conserved between the zebrafish embryo and human cells, we found that the oldest centrosome moves in a Rab11-dependent manner towards the cytokinetic bridge sometimes followed by the youngest. Rab11-endosomes are organized in a Rab11-GTP dependent manner at the mother centriole during pre-abscission, with Rab11 endosomes at the oldest centrosome being more mobile compared with the youngest. The GTPase activity of Rab11 is necessary for the centrosome protein, Pericentrin, to be enriched at the centrosome. Reduction in Pericentrin expression or optogenetic disruption of Rab11-endosome function inhibited both centrosome movement towards the cytokinetic bridge and abscission, resulting in daughter cells prone to being binucleated and/or having supernumerary centrosomes. These studies suggest that Rab11-endosomes contribute to centrosome function during pre-abscission by regulating Pericentrin organization resulting in appropriate centrosome movement towards the cytokinetic bridge and subsequent abscission.