TY - JOUR T1 - Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202201371 VL - 5 IS - 5 SP - e202201371 AU - Aneesha Kohli AU - Lucie Sauerhering AU - Sarah K Fehling AU - Kevin Klann AU - Helmut Geiger AU - Stephan Becker AU - Benjamin Koch AU - Patrick C Baer AU - Thomas Strecker AU - Christian Münch Y1 - 2022/05/01 UR - https://www.life-science-alliance.org/content/5/5/e202201371.abstract N2 - Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication. ER -