RT Journal Article SR Electronic T1 Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e202201371 DO 10.26508/lsa.202201371 VO 5 IS 5 A1 Aneesha Kohli A1 Lucie Sauerhering A1 Sarah K Fehling A1 Kevin Klann A1 Helmut Geiger A1 Stephan Becker A1 Benjamin Koch A1 Patrick C Baer A1 Thomas Strecker A1 Christian Münch YR 2022 UL https://www.life-science-alliance.org/content/5/5/e202201371.abstract AB Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication.