TY - JOUR T1 - Single-domain antibody screening by <em>is</em>PLA-seq JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202101115 VL - 5 IS - 1 SP - e202101115 AU - Yueyuan Yin AU - Fei Yan AU - Ruimin Zhou AU - Mingchen Li AU - Jinyi Ma AU - Zhe Liu AU - Zhenyi Ma Y1 - 2022/01/01 UR - https://www.life-science-alliance.org/content/5/1/e202101115.abstract N2 - Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular and single-cell resolution. Based on the sequence of complementarity-determining region 3 (CDR3), the recombinant sdAb can be produced for in vitro and in vivo utilities. This method provides a general means to identify the functional measure of sdAb and its complementary epitopes and its potential applications to investigate cellular processes. ER -