RT Journal Article
SR Electronic
T1 Single-domain antibody screening by <em>is</em>PLA-seq
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202101115
DO 10.26508/lsa.202101115
VO 5
IS 1
A1 Yueyuan Yin
A1 Fei Yan
A1 Ruimin Zhou
A1 Mingchen Li
A1 Jinyi Ma
A1 Zhe Liu
A1 Zhenyi Ma
YR 2022
UL https://www.life-science-alliance.org/content/5/1/e202101115.abstract
AB Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular and single-cell resolution. Based on the sequence of complementarity-determining region 3 (CDR3), the recombinant sdAb can be produced for in vitro and in vivo utilities. This method provides a general means to identify the functional measure of sdAb and its complementary epitopes and its potential applications to investigate cellular processes.