PT  - JOURNAL ARTICLE
AU  - Yueyuan Yin
AU  - Fei Yan
AU  - Ruimin Zhou
AU  - Mingchen Li
AU  - Jinyi Ma
AU  - Zhe Liu
AU  - Zhenyi Ma
TI  - Single-domain antibody screening by <em>is</em>PLA-seq
AID  - 10.26508/lsa.202101115
DP  - 2022 Jan 01
TA  - Life Science Alliance
PG  - e202101115
VI  - 5
IP  - 1
4099  - https://www.life-science-alliance.org/content/5/1/e202101115.short
4100  - https://www.life-science-alliance.org/content/5/1/e202101115.full
SO  - Life Sci. Alliance2022 Jan 01; 5
AB  - Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular and single-cell resolution. Based on the sequence of complementarity-determining region 3 (CDR3), the recombinant sdAb can be produced for in vitro and in vivo utilities. This method provides a general means to identify the functional measure of sdAb and its complementary epitopes and its potential applications to investigate cellular processes.