RT Journal Article SR Electronic T1 The DREAM complex represses growth in response to DNA damage in Arabidopsis JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e202101141 DO 10.26508/lsa.202101141 VO 4 IS 12 A1 Lucas Lang A1 Aladár Pettkó-Szandtner A1 Hasibe Tunçay Elbaşı A1 Hirotomo Takatsuka A1 Yuji Nomoto A1 Ahmad Zaki A1 Stefan Dorokhov A1 Geert De Jaeger A1 Dominique Eeckhout A1 Masaki Ito A1 Zoltán Magyar A1 László Bögre A1 Maren Heese A1 Arp Schnittger YR 2021 UL https://www.life-science-alliance.org/content/4/12/e202101141.abstract AB The DNA of all organisms is constantly damaged by physiological processes and environmental conditions. Upon persistent damage, plant growth and cell proliferation are reduced. Based on previous findings that RBR1, the only Arabidopsis homolog of the mammalian tumor suppressor gene retinoblastoma, plays a key role in the DNA damage response in plants, we unravel here the network of RBR1 interactors under DNA stress conditions. This led to the identification of homologs of every DREAM component in Arabidopsis, including previously not recognized homologs of LIN52. Interestingly, we also discovered NAC044, a mediator of DNA damage response in plants and close homolog of the major DNA damage regulator SOG1, to directly interact with RBR1 and the DREAM component LIN37B. Consistently, not only mutants in NAC044 but also the double mutant of the two LIN37 homologs and mutants for the DREAM component E2FB showed reduced sensitivities to DNA-damaging conditions. Our work indicates the existence of multiple DREAM complexes that work in conjunction with NAC044 to mediate growth arrest after DNA damage.