TY - JOUR T1 - Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202101222 VL - 4 IS - 12 SP - e202101222 AU - Jayant Asthana AU - Nicholas I Cade AU - Davide Normanno AU - Wei Ming Lim AU - Thomas Surrey Y1 - 2021/12/01 UR - https://www.life-science-alliance.org/content/4/12/e202101222.abstract N2 - During mitosis, the spindle undergoes morphological and dynamic changes. It reorganizes at the onset of the anaphase when the antiparallel bundler PRC1 accumulates and recruits central spindle proteins to the midzone. Little is known about how the dynamic properties of the central spindle change during its morphological changes in human cells. Using gene editing, we generated human cells that express from their endogenous locus fluorescent PRC1 and EB1 to quantify their native spindle distribution and binding/unbinding turnover. EB1 plus end tracking revealed a general slowdown of microtubule growth, whereas PRC1, similar to its yeast orthologue Ase1, binds increasingly strongly to compacting antiparallel microtubule overlaps. KIF4A and CLASP1 bind more dynamically to the central spindle, but also show slowing down turnover. These results show that the central spindle gradually becomes more stable during mitosis, in agreement with a recent “bundling, sliding, and compaction” model of antiparallel midzone bundle formation in the central spindle during late mitosis. ER -