TY - JOUR T1 - A role for Rad5 in ribonucleoside monophosphate (rNMP) tolerance JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000966 VL - 4 IS - 10 SP - e202000966 AU - Menattallah Elserafy AU - Iman El-Shiekh AU - Dalia Fleifel AU - Reham Atteya AU - Abdelrahman AlOkda AU - Mohamed M Abdrabbou AU - Mostafa Nasr AU - Sherif F El-Khamisy Y1 - 2021/10/01 UR - https://www.life-science-alliance.org/content/4/10/e202000966.abstract N2 - Ribonucleoside monophosphate (rNMP) incorporation in genomic DNA poses a significant threat to genomic integrity. In addition to repair, DNA damage tolerance mechanisms ensure replication progression upon encountering unrepaired lesions. One player in the tolerance mechanism is Rad5, which is an E3 ubiquitin ligase and helicase. Here, we report a new role for yeast Rad5 in tolerating rNMP incorporation, in the absence of the bona fide ribonucleotide excision repair pathway via RNase H2. This role of Rad5 is further highlighted after replication stress induced by hydroxyurea or by increasing rNMP genomic burden using a mutant DNA polymerase (Pol ε - Pol2-M644G). We further demonstrate the importance of the ATPase and ubiquitin ligase domains of Rad5 in rNMP tolerance. These findings suggest a similar role for the human Rad5 homologues helicase-like transcription factor (HLTF) and SNF2 Histone Linker PHD RING Helicase (SHPRH) in rNMP tolerance, which may impact the response of cancer cells to replication stress-inducing therapeutics. ER -