TY - JOUR T1 - Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cells JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000987 VL - 4 IS - 9 SP - e202000987 AU - Yelena Sargsyan AU - Uta Bickmeyer AU - Christine S Gibhardt AU - Katrin Streckfuss-Bömeke AU - Ivan Bogeski AU - Sven Thoms Y1 - 2021/09/01 UR - https://www.life-science-alliance.org/content/4/9/e202000987.abstract N2 - Peroxisomes communicate with other cellular compartments by transfer of various metabolites. However, whether peroxisomes are sites for calcium handling and exchange has remained contentious. Here we generated sensors for assessment of peroxisomal calcium and applied them for single cell-based calcium imaging in HeLa cells and cardiomyocytes. We found that peroxisomes in HeLa cells take up calcium upon depletion of intracellular calcium stores and upon calcium influx across the plasma membrane. Furthermore, we show that peroxisomes of neonatal rat cardiomyocytes and human induced pluripotent stem cell–derived cardiomyocytes can take up calcium. Our results indicate that peroxisomal and cytosolic calcium signals are tightly interconnected both in HeLa cells and in cardiomyocytes. Cardiac peroxisomes take up calcium on beat-to-beat basis. Hence, peroxisomes may play an important role in shaping cellular calcium dynamics of cardiomyocytes. ER -