PT  - JOURNAL ARTICLE
AU  - Yelena Sargsyan
AU  - Uta Bickmeyer
AU  - Christine S Gibhardt
AU  - Katrin Streckfuss-Bömeke
AU  - Ivan Bogeski
AU  - Sven Thoms
TI  - Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cells
AID  - 10.26508/lsa.202000987
DP  - 2021 Sep 01
TA  - Life Science Alliance
PG  - e202000987
VI  - 4
IP  - 9
4099  - https://www.life-science-alliance.org/content/4/9/e202000987.short
4100  - https://www.life-science-alliance.org/content/4/9/e202000987.full
SO  - Life Sci. Alliance2021 Sep 01; 4
AB  - Peroxisomes communicate with other cellular compartments by transfer of various metabolites. However, whether peroxisomes are sites for calcium handling and exchange has remained contentious. Here we generated sensors for assessment of peroxisomal calcium and applied them for single cell-based calcium imaging in HeLa cells and cardiomyocytes. We found that peroxisomes in HeLa cells take up calcium upon depletion of intracellular calcium stores and upon calcium influx across the plasma membrane. Furthermore, we show that peroxisomes of neonatal rat cardiomyocytes and human induced pluripotent stem cell–derived cardiomyocytes can take up calcium. Our results indicate that peroxisomal and cytosolic calcium signals are tightly interconnected both in HeLa cells and in cardiomyocytes. Cardiac peroxisomes take up calcium on beat-to-beat basis. Hence, peroxisomes may play an important role in shaping cellular calcium dynamics of cardiomyocytes.