RT Journal Article SR Electronic T1 Dynamic changes in RNA–protein interactions and RNA secondary structure in mammalian erythropoiesis JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e202000659 DO 10.26508/lsa.202000659 VO 4 IS 9 A1 Mengge Shan A1 Xinjun Ji A1 Kevin Janssen A1 Ian M Silverman A1 Jesse Humenik A1 Ben A Garcia A1 Stephen A Liebhaber A1 Brian D Gregory YR 2021 UL https://www.life-science-alliance.org/content/4/9/e202000659.abstract AB Two features of eukaryotic RNA molecules that regulate their post-transcriptional fates are RNA secondary structure and RNA-binding protein (RBP) interaction sites. However, a comprehensive global overview of the dynamic nature of these sequence features during erythropoiesis has never been obtained. Here, we use our ribonuclease-mediated structure and RBP-binding site mapping approach to reveal the global landscape of RNA secondary structure and RBP–RNA interaction sites and the dynamics of these features during this important developmental process. We identify dynamic patterns of RNA secondary structure and RBP binding throughout the process and determine a set of corresponding protein-bound sequence motifs along with their dynamic structural and RBP-binding contexts. Finally, using these dynamically bound sequences, we identify a number of RBPs that have known and putative key functions in post-transcriptional regulation during mammalian erythropoiesis. In total, this global analysis reveals new post-transcriptional regulators of mammalian blood cell development.