RT Journal Article
SR Electronic
T1 Small intestinal flora graft alters fecal flora, stool, cytokines and mood status in healthy mice
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202101039
DO 10.26508/lsa.202101039
VO 4
IS 9
A1 Yinyin Xie
A1 Linyang Song
A1 Junhua Yang
A1 Taoqi Tao
A1 Jing Yu
A1 Jingrong Shi
A1 Xiaobao Jin
YR 2021
UL https://www.life-science-alliance.org/content/4/9/e202101039.abstract
AB Fecal microbiota transplantation is widely used. Large intestinal microbiota (LIM) is more similar to fecal microbiota than small intestinal microbiota (SIM). The SIM communities are very different from those of LIM. Therefore, SIM transplantation (SIMT) and LIM transplantation (LIMT) might exert different influences. Here, healthy adult male C57Bl/6 mice received intragastric SIMT, LIMT, or sterile PBS administration. Microbiota graft samples were collected from small/large intestine of healthy mice of the same age, sex, and strain background. Compared with PBS treatment, SIMT increased pellet number, stool wet weight, and stool water percentage; induced a fecal microbiota profile shift toward the microbial composition of the SIM graft; induced a systemic anti-inflammatory cytokines profile; and ameliorated depressive-like behaviors in recipients. LIMT, however, induced merely a slight alteration in fecal microbial composition and no significant influence on the other aspects. In sum, SIMT, rather than LIMT, affected defecation features, fecal microbial composition, cytokines profile, and depressive-like behaviors in healthy mice. This study reveals the different effects of SIMT and LIMT, providing an interesting clue for further researches involving gut microbial composition change.