PT  - JOURNAL ARTICLE
AU  - Xie, Yinyin
AU  - Song, Linyang
AU  - Yang, Junhua
AU  - Tao, Taoqi
AU  - Yu, Jing
AU  - Shi, Jingrong
AU  - Jin, Xiaobao
TI  - Small intestinal flora graft alters fecal flora, stool, cytokines and mood status in healthy mice
AID  - 10.26508/lsa.202101039
DP  - 2021 Sep 01
TA  - Life Science Alliance
PG  - e202101039
VI  - 4
IP  - 9
4099  - http://www.life-science-alliance.org/content/4/9/e202101039.short
4100  - http://www.life-science-alliance.org/content/4/9/e202101039.full
SO  - Life Sci. Alliance2021 Sep 01; 4
AB  - Fecal microbiota transplantation is widely used. Large intestinal microbiota (LIM) is more similar to fecal microbiota than small intestinal microbiota (SIM). The SIM communities are very different from those of LIM. Therefore, SIM transplantation (SIMT) and LIM transplantation (LIMT) might exert different influences. Here, healthy adult male C57Bl/6 mice received intragastric SIMT, LIMT, or sterile PBS administration. Microbiota graft samples were collected from small/large intestine of healthy mice of the same age, sex, and strain background. Compared with PBS treatment, SIMT increased pellet number, stool wet weight, and stool water percentage; induced a fecal microbiota profile shift toward the microbial composition of the SIM graft; induced a systemic anti-inflammatory cytokines profile; and ameliorated depressive-like behaviors in recipients. LIMT, however, induced merely a slight alteration in fecal microbial composition and no significant influence on the other aspects. In sum, SIMT, rather than LIMT, affected defecation features, fecal microbial composition, cytokines profile, and depressive-like behaviors in healthy mice. This study reveals the different effects of SIMT and LIMT, providing an interesting clue for further researches involving gut microbial composition change.