TY - JOUR T1 - Distinct mechanisms mediate X chromosome dosage compensation in <em>Anopheles</em> and <em>Drosophila</em> JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000996 VL - 4 IS - 9 SP - e202000996 AU - Claudia Isabelle Keller Valsecchi AU - Eric Marois AU - M Felicia Basilicata AU - Plamen Georgiev AU - Asifa Akhtar Y1 - 2021/09/01 UR - https://www.life-science-alliance.org/content/4/9/e202000996.abstract N2 - Sex chromosomes induce potentially deleterious gene expression imbalances that are frequently corrected by dosage compensation (DC). Three distinct molecular strategies to achieve DC have been previously described in nematodes, fruit flies, and mammals. Is this a consequence of distinct genomes, functional or ecological constraints, or random initial commitment to an evolutionary trajectory? Here, we study DC in the malaria mosquito Anopheles gambiae. The Anopheles and Drosophila X chromosomes evolved independently but share a high degree of homology. We find that Anopheles achieves DC by a mechanism distinct from the Drosophila MSL complex–histone H4 lysine 16 acetylation pathway. CRISPR knockout of Anopheles msl-2 leads to embryonic lethality in both sexes. Transcriptome analyses indicate that this phenotype is not a consequence of defective X chromosome DC. By immunofluorescence and ChIP, H4K16ac does not preferentially enrich on the male X. Instead, the mosquito MSL pathway regulates conserved developmental genes. We conclude that a novel mechanism confers X chromosome up-regulation in Anopheles. Our findings highlight the pluralism of gene-dosage buffering mechanisms even under similar genomic and functional constraints. ER -