PT - JOURNAL ARTICLE AU - Beatrice Terni AU - Artur Llobet TI - Axon terminals control endolysosome diffusion to support synaptic remodelling AID - 10.26508/lsa.202101105 DP - 2021 Aug 01 TA - Life Science Alliance PG - e202101105 VI - 4 IP - 8 4099 - https://www.life-science-alliance.org/content/4/8/e202101105.short 4100 - https://www.life-science-alliance.org/content/4/8/e202101105.full SO - Life Sci. Alliance2021 Aug 01; 4 AB - Endolysosomes are acidic organelles formed by the fusion of endosomes with lysosomes. In the presynaptic compartment they contribute to protein homeostasis, the maintenance of vesicle pools and synaptic stability. Here, we evaluated the mobility of endolysosomes found in axon terminals of olfactory sensory neurons of Xenopus tropicalis tadpoles. F-actin restricts the motion of these presynaptic acidic organelles which is characterized by a diffusion coefficient of 6.7 × 10−3 μm2·s−1. Local injection of secreted protein acidic and rich in cysteine (SPARC) in the glomerular layer of the olfactory bulb disrupts the structure of synaptic F-actin patches and increases the presence and mobility of endolysosomal organelles found in axon terminals. The increased motion of endolysosomes is localized to the presynaptic compartment and does not promote their access to axonal regions for retrograde transportation to the cell body. Local activation of synaptic degradation mechanisms mediated by SPARC coincides with a loss of the ability of tadpoles to detect waterborne odorants. Together, these observations show that the diffusion of presynaptic endolysosomes increases during conditions of synaptic remodelling to support their local degradative activity.