TY - JOUR T1 - UVB mutagenesis differs in <em>Nras</em>- and <em>Braf</em>-mutant mouse models of melanoma JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202101135 VL - 4 IS - 9 SP - e202101135 AU - Robert L Bowman AU - Rebecca C Hennessey AU - Tirzah J Weiss AU - David A Tallman AU - Emma R Crawford AU - Brandon M Murphy AU - Amy Webb AU - Souhui Zhang AU - Krista MD La Perle AU - Craig J Burd AU - Ross L Levine AU - A Hunter Shain AU - Christin E Burd Y1 - 2021/09/01 UR - https://www.life-science-alliance.org/content/4/9/e202101135.abstract N2 - BRAF-mutant melanomas are more likely than NRAS-mutant melanomas to arise in anatomical locations protected from chronic sun damage. We hypothesized that this discrepancy in tumor location is a consequence of the differential sensitivity of BRAF and NRAS-mutant melanocytes to ultraviolet light (UV)-mediated carcinogenesis. We tested this hypothesis by comparing the mutagenic consequences of a single neonatal, ultraviolet-AI (UVA; 340–400 nm) or ultraviolet-B (UVB; 280–390 nm) exposure in mouse models heterozygous for mutant Braf or homozygous for mutant Nras. Tumor onset was accelerated by UVB, but not UVA, and the resulting melanomas contained recurrent mutations affecting the RING domain of MAP3K1 and Actin-binding domain of Filamin A. Melanomas from UVB-irradiated, Braf-mutant mice averaged twice as many single-nucleotide variants and five times as many dipyrimidine variants than tumors from similarly irradiated Nras-mutant mice. A mutational signature discovered in UVB-accelerated tumors mirrored COSMIC signatures associated with human skin cancer and was more prominent in Braf- than Nras-mutant murine melanomas. These data show that a single UVB exposure yields a greater burden of mutations in murine tumors driven by oncogenic Braf. ER -