RT Journal Article
SR Electronic
T1 Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egress
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000910
DO 10.26508/lsa.202000910
VO 4
IS 8
A1 Moona Huttunen
A1 Jerzy Samolej
A1 Robert J Evans
A1 Artur Yakimovich
A1 Ian J White
A1 Janos Kriston-Vizi
A1 Juan Martin-Serrano
A1 Wesley I Sundquist
A1 Eva-Maria Frickel
A1 Jason Mercer
YR 2021
UL https://www.life-science-alliance.org/content/4/8/e202000910.abstract
AB Poxvirus egress is a complex process whereby cytoplasmic single membrane–bound virions are wrapped in a cell-derived double membrane. These triple-membrane particles, termed intracellular enveloped virions (IEVs), are released from infected cells by fusion. Whereas the wrapping double membrane is thought to be derived from virus-modified trans-Golgi or early endosomal cisternae, the cellular factors that regulate virus wrapping remain largely undefined. To identify cell factors required for this process the prototypic poxvirus, vaccinia virus (VACV), was subjected to an RNAi screen directed against cellular membrane-trafficking proteins. Focusing on the endosomal sorting complexes required for transport (ESCRT), we demonstrate that ESCRT-III and VPS4 are required for packaging of virus into multivesicular bodies (MVBs). EM-based characterization of MVB-IEVs showed that they account for half of IEV production indicating that MVBs are a second major source of VACV wrapping membrane. These data support a model whereby, in addition to cisternae-based wrapping, VACV hijacks ESCRT-mediated MVB formation to facilitate virus egress and spread.