RT Journal Article
SR Electronic
T1 Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000945
DO 10.26508/lsa.202000945
VO 4
IS 7
A1 Tatsuo Suzuki
A1 Nobuo Terada
A1 Shigeki Higashiyama
A1 Kiyokazu Kametani
A1 Yoshinori Shirai
A1 Mamoru Honda
A1 Tsutomu Kai
A1 Weidong Li
A1 Katsuhiko Tabuchi
YR 2021
UL https://www.life-science-alliance.org/content/4/7/e202000945.abstract
AB A purification protocol was developed to identify and analyze the component proteins of a postsynaptic density (PSD) lattice, a core structure of the PSD of excitatory synapses in the central nervous system. “Enriched”- and “lean”-type PSD lattices were purified by synaptic plasma membrane treatment to identify the protein components by comprehensive shotgun mass spectrometry and group them into minimum essential cytoskeleton (MEC) and non-MEC components. Tubulin was found to be a major component of the MEC, with non-microtubule tubulin widely distributed on the purified PSD lattice. The presence of tubulin in and around PSDs was verified by post-embedding immunogold labeling EM of cerebral cortex. Non-MEC proteins included various typical scaffold/adaptor PSD proteins and other class PSD proteins. Thus, this study provides a new PSD lattice model consisting of non-microtubule tubulin-based backbone and various non-MEC proteins. Our findings suggest that tubulin is a key component constructing the backbone and that the associated components are essential for the versatile functions of the PSD.