PT - JOURNAL ARTICLE AU - Mayeux, Géraldine AU - Gayet, Landry AU - Liguori, Lavinia AU - Odier, Marine AU - Martin, Donald K AU - Cortès, Sandra AU - Schaack, Béatrice AU - Lenormand, Jean-Luc TI - Cell-free expression of the outer membrane protein OprF of <em>Pseudomonas aeruginosa</em> for vaccine purposes AID - 10.26508/lsa.202000958 DP - 2021 Jun 01 TA - Life Science Alliance PG - e202000958 VI - 4 IP - 6 4099 - https://www.life-science-alliance.org/content/4/6/e202000958.short 4100 - https://www.life-science-alliance.org/content/4/6/e202000958.full SO - Life Sci. Alliance2021 Jun 01; 4 AB - Pseudomonas aeruginosa is the second-leading cause of nosocomial infections and pneumonia in hospitals. Because of its extraordinary capacity for developing resistance to antibiotics, treating infections by Pseudomonas is becoming a challenge, lengthening hospital stays, and increasing medical costs and mortality. The outer membrane protein OprF is a well-conserved and immunogenic porin playing an important role in quorum sensing and in biofilm formation. Here, we used a bacterial cell-free expression system to reconstitute OprF under its native forms in liposomes and we demonstrated that the resulting OprF proteoliposomes can be used as a fully functional recombinant vaccine against P. aeruginosa. Remarkably, we showed that our system promotes the folding of OprF into its active open oligomerized state as well as the formation of mega-pores. Our approach thus represents an easy and efficient way for producing bacterial membrane antigens exposing native epitopes for vaccine purposes.