PT  - JOURNAL ARTICLE
AU  - Géraldine Mayeux
AU  - Landry Gayet
AU  - Lavinia Liguori
AU  - Marine Odier
AU  - Donald K Martin
AU  - Sandra Cortès
AU  - Béatrice Schaack
AU  - Jean-Luc Lenormand
TI  - Cell-free expression of the outer membrane protein OprF of <em>Pseudomonas aeruginosa</em> for vaccine purposes
AID  - 10.26508/lsa.202000958
DP  - 2021 Jun 01
TA  - Life Science Alliance
PG  - e202000958
VI  - 4
IP  - 6
4099  - https://www.life-science-alliance.org/content/4/6/e202000958.short
4100  - https://www.life-science-alliance.org/content/4/6/e202000958.full
SO  - Life Sci. Alliance2021 Jun 01; 4
AB  - Pseudomonas aeruginosa is the second-leading cause of nosocomial infections and pneumonia in hospitals. Because of its extraordinary capacity for developing resistance to antibiotics, treating infections by Pseudomonas is becoming a challenge, lengthening hospital stays, and increasing medical costs and mortality. The outer membrane protein OprF is a well-conserved and immunogenic porin playing an important role in quorum sensing and in biofilm formation. Here, we used a bacterial cell-free expression system to reconstitute OprF under its native forms in liposomes and we demonstrated that the resulting OprF proteoliposomes can be used as a fully functional recombinant vaccine against P. aeruginosa. Remarkably, we showed that our system promotes the folding of OprF into its active open oligomerized state as well as the formation of mega-pores. Our approach thus represents an easy and efficient way for producing bacterial membrane antigens exposing native epitopes for vaccine purposes.