RT Journal Article
SR Electronic
T1 Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000840
DO 10.26508/lsa.202000840
VO 4
IS 4
A1 Kahraman, Sevim
A1 Manna, Debasish
A1 Dirice, Ercument
A1 Maji, Basudeb
A1 Small, Jonnell
A1 Wagner, Bridget K
A1 Choudhary, Amit
A1 Kulkarni, Rohit N
YR 2021
UL http://www.life-science-alliance.org/content/4/4/e202000840.abstract
AB Highly sensitive approaches to target insulin-expressing cells would allow more effective imaging, sorting, and analysis of pancreatic β-cells. Here, we introduce the use of a reaction-based probe, diacetylated Zinpyr1 (DA-ZP1), to image pancreatic β-cells and β-like cells derived from human pluripotent stem cells. We harness the high intracellular zinc concentration of β-cells to induce a fluorescence signal in cells after administration of DA-ZP1. Given its specificity and rapid uptake by cells, we used DA-ZP1 to purify live stem cell-derived β-like cells as confirmed by immunostaining analysis. We tested the ability of DA-ZP1 to image transplanted human islet grafts and endogenous mouse pancreatic islets in vivo after its systemic administration into mice. Thus, DA-ZP1 enables purification of insulin-secreting β-like cells for downstream applications, such as functional studies, gene-expression, and cell–cell interaction analyses and can be used to label engrafted human islets and endogenous mouse islets in vivo.