RT Journal Article
SR Electronic
T1 A novel role of DNA polymerase λ in translesion synthesis in conjunction with DNA polymerase ζ
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000900
DO 10.26508/lsa.202000900
VO 4
IS 4
A1 Yoon, Jung-Hoon
A1 Basu, Debashree
A1 Sellamuthu, Karthi
A1 Johnson, Robert E
A1 Prakash, Satya
A1 Prakash, Louise
YR 2021
UL https://www.life-science-alliance.org/content/4/4/e202000900.abstract
AB By extending synthesis opposite from a diverse array of DNA lesions, DNA polymerase (Pol) ζ performs a crucial role in translesion synthesis (TLS). In yeast and cancer cells, Rev1 functions as an indispensable scaffolding component of Polζ and it imposes highly error-prone TLS upon Polζ. However, for TLS that occurs during replication in normal human cells, Rev1 functions instead as a scaffolding component of Pols η, ι, and κ and Rev1-dependent TLS by these Pols operates in a predominantly error-free manner. The lack of Rev1 requirement for Polζ function in TLS in normal cells suggested that some other protein substitutes for this Rev1 role. Here, we identify a novel role of Polλ as an indispensable scaffolding component of Polζ. TLS studies opposite a number of DNA lesions support the conclusion that as an integral component, Polλ adapts Polζ-dependent TLS to operate in a predominantly error-free manner in human cells, essential for genome integrity and cellular homeostasis.