PT  - JOURNAL ARTICLE
AU  - Yoon, Jung-Hoon
AU  - Basu, Debashree
AU  - Sellamuthu, Karthi
AU  - Johnson, Robert E
AU  - Prakash, Satya
AU  - Prakash, Louise
TI  - A novel role of DNA polymerase λ in translesion synthesis in conjunction with DNA polymerase ζ
AID  - 10.26508/lsa.202000900
DP  - 2021 Apr 01
TA  - Life Science Alliance
PG  - e202000900
VI  - 4
IP  - 4
4099  - https://www.life-science-alliance.org/content/4/4/e202000900.short
4100  - https://www.life-science-alliance.org/content/4/4/e202000900.full
SO  - Life Sci. Alliance2021 Apr 01; 4
AB  - By extending synthesis opposite from a diverse array of DNA lesions, DNA polymerase (Pol) ζ performs a crucial role in translesion synthesis (TLS). In yeast and cancer cells, Rev1 functions as an indispensable scaffolding component of Polζ and it imposes highly error-prone TLS upon Polζ. However, for TLS that occurs during replication in normal human cells, Rev1 functions instead as a scaffolding component of Pols η, ι, and κ and Rev1-dependent TLS by these Pols operates in a predominantly error-free manner. The lack of Rev1 requirement for Polζ function in TLS in normal cells suggested that some other protein substitutes for this Rev1 role. Here, we identify a novel role of Polλ as an indispensable scaffolding component of Polζ. TLS studies opposite a number of DNA lesions support the conclusion that as an integral component, Polλ adapts Polζ-dependent TLS to operate in a predominantly error-free manner in human cells, essential for genome integrity and cellular homeostasis.