RT Journal Article
SR Electronic
T1 FAM83F regulates canonical Wnt signalling through an interaction with CK1α
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000805
DO 10.26508/lsa.202000805
VO 4
IS 2
A1 Karen Dunbar
A1 Rebecca A Jones
A1 Kevin Dingwell
A1 Thomas J Macartney
A1 James C Smith
A1 Gopal P Sapkota
YR 2021
UL https://www.life-science-alliance.org/content/4/2/e202000805.abstract
AB The function of the FAM83F protein, like the functions of many members of the FAM83 family, is poorly understood. Here, we show that injection of Fam83f mRNA into Xenopus embryos causes axis duplication, a phenotype indicative of enhanced Wnt signalling. Consistent with this, overexpression of FAM83F activates Wnt signalling, whereas ablation of FAM83F from human colorectal cancer (CRC) cells attenuates it. We demonstrate that FAM83F is farnesylated and interacts and co-localises with CK1α at the plasma membrane. This interaction with CK1α is essential for FAM83F to activate Wnt signalling, and FAM83F mutants that do not interact with CK1α fail to induce axis duplication in Xenopus embryos and to activate Wnt signalling in cells. FAM83F acts upstream of GSK-3β because the attenuation of Wnt signalling caused by loss of FAM83F can be rescued by GSK-3 inhibition. Introduction of a farnesyl-deficient mutant of FAM83F in cells through CRISPR/Cas9 genome editing redirects the FAM83F–CK1α complex away from the plasma membrane and significantly attenuates Wnt signalling, indicating that FAM83F exerts its effects on Wnt signalling at the plasma membrane.