TY - JOUR T1 - SCF<sup>Cdc4</sup> ubiquitin ligase regulates synaptonemal complex formation during meiosis JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000933 VL - 4 IS - 2 SP - e202000933 AU - Zhihui Zhu AU - Mohammad Bani Ismail AU - Miki Shinohara AU - Akira Shinohara Y1 - 2021/02/01 UR - https://www.life-science-alliance.org/content/4/2/e202000933.abstract N2 - Homologous chromosomes pair with each other during meiosis, culminating in the formation of the synaptonemal complex (SC), which is coupled with meiotic recombination. In this study, we showed that a meiosis-specific depletion mutant of a cullin (Cdc53) in the SCF (Skp-Cullin-F-box) ubiquitin ligase, which plays a critical role in cell cycle regulation during mitosis, is deficient in SC formation. However, the mutant is proficient in forming crossovers, indicating the uncoupling of meiotic recombination with SC formation in the mutant. Furthermore, the deletion of the PCH2 gene encoding a meiosis-specific AAA+ ATPase suppresses SC-assembly defects induced by CDC53 depletion. On the other hand, the pch2 cdc53 double mutant is defective in meiotic crossover formation, suggesting the assembly of SC with unrepaired DNA double-strand breaks. A temperature-sensitive mutant of CDC4, which encodes an F-box protein of SCF, shows meiotic defects similar to those of the CDC53-depletion mutant. These results suggest that SCFCdc4, probably SCFCdc4-dependent protein ubiquitylation, regulates and collaborates with Pch2 in SC assembly and meiotic recombination. ER -