RT Journal Article
SR Electronic
T1 Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000924
DO 10.26508/lsa.202000924
VO 4
IS 1
A1 Karlina, Ruth
A1 Lutter, Dominik
A1 Miok, Viktorian
A1 Fischer, David
A1 Altun, Irem
A1 Schöttl, Theresa
A1 Schorpp, Kenji
A1 Israel, Andreas
A1 Cero, Cheryl
A1 Johnson, James W
A1 Kapser-Fischer, Ingrid
A1 Böttcher, Anika
A1 Keipert, Susanne
A1 Feuchtinger, Annette
A1 Graf, Elisabeth
A1 Strom, Tim
A1 Walch, Axel
A1 Lickert, Heiko
A1 Walzthoeni, Thomas
A1 Heinig, Matthias
A1 Theis, Fabian J
A1 García-Cáceres, Cristina
A1 Cypess, Aaron M
A1 Ussar, Siegfried
YR 2021
UL http://www.life-science-alliance.org/content/4/1/e202000924.abstract
AB Brown adipose tissue (BAT) plays an important role in the regulation of body weight and glucose homeostasis. Although increasing evidence supports white adipose tissue heterogeneity, little is known about heterogeneity within murine BAT. Recently, UCP1 high and low expressing brown adipocytes were identified, but a developmental origin of these subtypes has not been studied. To obtain more insights into brown preadipocyte heterogeneity, we use single-cell RNA sequencing of the BAT stromal vascular fraction of C57/BL6 mice and characterize brown preadipocyte and adipocyte clonal cell lines. Statistical analysis of gene expression profiles from brown preadipocyte and adipocyte clones identify markers distinguishing brown adipocyte subtypes. We confirm the presence of distinct brown adipocyte populations in vivo using the markers EIF5, TCF25, and BIN1. We also demonstrate that loss of Bin1 enhances UCP1 expression and mitochondrial respiration, suggesting that BIN1 marks dormant brown adipocytes. The existence of multiple brown adipocyte subtypes suggests distinct functional properties of BAT depending on its cellular composition, with potentially distinct functions in thermogenesis and the regulation of whole body energy homeostasis.