PT - JOURNAL ARTICLE AU - Jinbo Fan AU - Hui Jin AU - Bailey A Koch AU - Hong-Guo Yu TI - Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast AID - 10.26508/lsa.202000824 DP - 2020 Dec 01 TA - Life Science Alliance PG - e202000824 VI - 3 IP - 12 4099 - https://www.life-science-alliance.org/content/3/12/e202000824.short 4100 - https://www.life-science-alliance.org/content/3/12/e202000824.full SO - Life Sci. Alliance2020 Dec 01; 3 AB - The linker of the nucleoskeleton and cytoskeleton (LINC) complex is composed of two transmembrane proteins: the KASH domain protein localized to the outer nuclear membrane and the SUN domain protein to the inner nuclear membrane. In budding yeast, the sole SUN domain protein, Mps3, is thought to pair with either Csm4 or Mps2, two KASH-like proteins, to form two separate LINC complexes. Here, we show that Mps2 mediates the interaction between Csm4 and Mps3 to form a heterotrimeric telomere-associated LINC (t-LINC) complex in budding yeast meiosis. Mps2 binds to Csm4 and Mps3, and all three are localized to the telomere. Telomeric localization of Csm4 depends on both Mps2 and Mps3; in contrast, Mps2’s localization depends on Mps3 but not Csm4. Mps2-mediated t-LINC complex regulates telomere movement and meiotic recombination. By ectopically expressing CSM4 in vegetative yeast cells, we reconstitute the heterotrimeric t-LINC complex and demonstrate its ability to tether telomeres. Our findings therefore reveal the heterotrimeric composition of the t-LINC complex in budding yeast and have implications for understanding variant LINC complex formation.