PT  - JOURNAL ARTICLE
AU  - Meliha Mehmeti-Ajradini
AU  - Caroline Bergenfelz
AU  - Anna-Maria Larsson
AU  - Robert Carlsson
AU  - Kristian Riesbeck
AU  - Jonas Ahl
AU  - Helena Janols
AU  - Marlene Wullt
AU  - Anders Bredberg
AU  - Eva Källberg
AU  - Frida Björk Gunnarsdottir
AU  - Camilla Rydberg Millrud
AU  - Lisa Rydén
AU  - Gesine Paul
AU  - Niklas Loman
AU  - Jörgen Adolfsson
AU  - Ana Carneiro
AU  - Karin Jirström
AU  - Fredrika Killander
AU  - Daniel Bexell
AU  - Karin Leandersson
TI  - Human G-MDSCs are neutrophils at distinct maturation stages promoting tumor growth in breast cancer
AID  - 10.26508/lsa.202000893
DP  - 2020 Nov 01
TA  - Life Science Alliance
PG  - e202000893
VI  - 3
IP  - 11
4099  - https://www.life-science-alliance.org/content/3/11/e202000893.short
4100  - https://www.life-science-alliance.org/content/3/11/e202000893.full
SO  - Life Sci. Alliance2020 Nov 01; 3
AB  - Myeloid-derived suppressor cells (MDSCs) are known to contribute to immune evasion in cancer. However, the function of the human granulocytic (G)-MDSC subset during tumor progression is largely unknown, and there are no established markers for their identification in human tumor specimens. Using gene expression profiling, mass cytometry, and tumor microarrays, we here demonstrate that human G-MDSCs occur as neutrophils at distinct maturation stages, with a disease-specific profile. G-MDSCs derived from patients with metastatic breast cancer and malignant melanoma display a unique immature neutrophil profile, that is more similar to healthy donor neutrophils than to G-MDSCs from sepsis patients. Finally, we show that primary G-MDSCs from metastatic breast cancer patients co-transplanted with breast cancer cells, promote tumor growth, and affect vessel formation, leading to myeloid immune cell exclusion. Our findings reveal a role for human G-MDSC in tumor progression and have clinical implications also for targeted immunotherapy.