RT Journal Article SR Electronic T1 The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e202000720 DO 10.26508/lsa.202000720 VO 3 IS 10 A1 Tran, Tuan Minh A1 Ma, Zhiming A1 Triebl, Alexander A1 Nath, Sangeeta A1 Cheng, Yingying A1 Gong, Ben-Qiang A1 Han, Xiao A1 Wang, Junqi A1 Li, Jian-Feng A1 Wenk, Markus R A1 Torta, Federico A1 Mayor, Satyajit A1 Yang, Liang A1 Miao, Yansong YR 2020 UL http://www.life-science-alliance.org/content/3/10/e202000720.abstract AB Quorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (cis-11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. campestris can suppress pathogen-associated molecular pattern–triggered immunity (PTI) in Arabidopsis thaliana, mediated by flagellin-induced activation of flagellin receptor FLS2. The DSF-mediated attenuation of innate immunity results from the alteration of FLS2 nanoclusters and endocytic internalization of plasma membrane FLS2. DSF altered the lipid profile of Arabidopsis, with a particular increase in the phytosterol species, which impairs the general endocytosis pathway mediated by clathrin and FLS2 nano-clustering on the plasma membrane. The DSF effect on receptor dynamics and host immune responses could be entirely reversed by sterol removal. Together, our results highlighted the importance of sterol homeostasis to plasma membrane organization and demonstrate a novel mechanism by which pathogenic bacteria use their communicating molecule to manipulate pathogen-associated molecular pattern–triggered host immunity.