TY - JOUR T1 - Profiles of histidine-rich glycoprotein associate with age and risk of all-cause mortality JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000817 VL - 3 IS - 10 SP - e202000817 AU - Mun-Gwan Hong AU - Tea Dodig-Crnković AU - Xu Chen AU - Kimi Drobin AU - Woojoo Lee AU - Yunzhang Wang AU - Fredrik Edfors AU - David Kotol AU - Cecilia Engel Thomas AU - Ronald Sjöberg AU - Jacob Odeberg AU - Anders Hamsten AU - Angela Silveira AU - Per Hall AU - Peter Nilsson AU - Yudi Pawitan AU - Mathias Uhlén AU - Nancy L Pedersen AU - Sara Hägg AU - Patrik KE Magnusson AU - Jochen M Schwenk Y1 - 2020/10/01 UR - https://www.life-science-alliance.org/content/3/10/e202000817.abstract N2 - Despite recognizing aging as a common risk factor of many human diseases, little is known about its molecular traits. To identify age-associated proteins circulating in human blood, we screened 156 individuals aged 50–92 using exploratory and multiplexed affinity proteomics assays. Profiling eight additional study sets (N = 3,987), performing antibody validation, and conducting a meta-analysis revealed a consistent age association (P = 6.61 × 10−6) for circulating histidine-rich glycoprotein (HRG). Sequence variants of HRG influenced how the protein was recognized in the immunoassays. Indeed, only the HRG profiles affected by rs9898 were associated with age and predicted the risk of mortality (HR = 1.25 per SD; 95% CI = 1.12–1.39; P = 6.45 × 10−5) during a follow-up period of 8.5 yr after blood sampling (IQR = 7.7–9.3 yr). Our affinity proteomics analysis found associations between the particular molecular traits of circulating HRG with age and all-cause mortality. The distinct profiles of this multipurpose protein could serve as an accessible and informative indicator of the physiological processes related to biological aging. ER -