RT Journal Article
SR Electronic
T1 EBV renders B cells susceptible to HIV-1 in humanized mice
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000640
DO 10.26508/lsa.202000640
VO 3
IS 8
A1 Donal McHugh
A1 Renier Myburgh
A1 Nicole Caduff
A1 Michael Spohn
A1 Yik Lim Kok
A1 Christian W Keller
A1 Anita Murer
A1 Bithi Chatterjee
A1 Julia Rühl
A1 Christine Engelmann
A1 Obinna Chijioke
A1 Isaak Quast
A1 Mohaned Shilaih
A1 Victoria P Strouvelle
A1 Kathrin Neumann
A1 Thomas Menter
A1 Stephan Dirnhofer
A1 Janice KP Lam
A1 Kwai F Hui
A1 Simon Bredl
A1 Erika Schlaepfer
A1 Silvia Sorce
A1 Andrea Zbinden
A1 Riccarda Capaul
A1 Jan D Lünemann
A1 Adriano Aguzzi
A1 Alan KS Chiang
A1 Werner Kempf
A1 Alexandra Trkola
A1 Karin J Metzner
A1 Markus G Manz
A1 Adam Grundhoff
A1 Roberto F Speck
A1 Christian Münz
YR 2020
UL https://www.life-science-alliance.org/content/3/8/e202000640.abstract
AB HIV and EBV are human pathogens that cause a considerable burden to worldwide health. In combination, these viruses are linked to AIDS-associated lymphomas. We found that EBV, which transforms B cells, renders them susceptible to HIV-1 infection in a CXCR4 and CD4-dependent manner in vitro and that CXCR4-tropic HIV-1 integrates into the genome of these B cells with the same molecular profile as in autologous CD4+ T cells. In addition, we established a humanized mouse model to investigate the in vivo interactions of EBV and HIV-1 upon coinfection. The respective mice that reconstitute human immune system components upon transplantation with CD34+ human hematopoietic progenitor cells could recapitulate aspects of EBV and HIV immunobiology observed in dual-infected patients. Upon coinfection of humanized mice, EBV/HIV dual-infected B cells could be detected, but were susceptible to CD8+ T-cell–mediated immune control.