TY - JOUR T1 - EBV renders B cells susceptible to HIV-1 in humanized mice JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000640 VL - 3 IS - 8 SP - e202000640 AU - Donal McHugh AU - Renier Myburgh AU - Nicole Caduff AU - Michael Spohn AU - Yik Lim Kok AU - Christian W Keller AU - Anita Murer AU - Bithi Chatterjee AU - Julia Rühl AU - Christine Engelmann AU - Obinna Chijioke AU - Isaak Quast AU - Mohaned Shilaih AU - Victoria P Strouvelle AU - Kathrin Neumann AU - Thomas Menter AU - Stephan Dirnhofer AU - Janice KP Lam AU - Kwai F Hui AU - Simon Bredl AU - Erika Schlaepfer AU - Silvia Sorce AU - Andrea Zbinden AU - Riccarda Capaul AU - Jan D Lünemann AU - Adriano Aguzzi AU - Alan KS Chiang AU - Werner Kempf AU - Alexandra Trkola AU - Karin J Metzner AU - Markus G Manz AU - Adam Grundhoff AU - Roberto F Speck AU - Christian Münz Y1 - 2020/08/01 UR - https://www.life-science-alliance.org/content/3/8/e202000640.abstract N2 - HIV and EBV are human pathogens that cause a considerable burden to worldwide health. In combination, these viruses are linked to AIDS-associated lymphomas. We found that EBV, which transforms B cells, renders them susceptible to HIV-1 infection in a CXCR4 and CD4-dependent manner in vitro and that CXCR4-tropic HIV-1 integrates into the genome of these B cells with the same molecular profile as in autologous CD4+ T cells. In addition, we established a humanized mouse model to investigate the in vivo interactions of EBV and HIV-1 upon coinfection. The respective mice that reconstitute human immune system components upon transplantation with CD34+ human hematopoietic progenitor cells could recapitulate aspects of EBV and HIV immunobiology observed in dual-infected patients. Upon coinfection of humanized mice, EBV/HIV dual-infected B cells could be detected, but were susceptible to CD8+ T-cell–mediated immune control. ER -