PT - JOURNAL ARTICLE AU - McHugh, Donal AU - Myburgh, Renier AU - Caduff, Nicole AU - Spohn, Michael AU - Kok, Yik Lim AU - Keller, Christian W AU - Murer, Anita AU - Chatterjee, Bithi AU - Rühl, Julia AU - Engelmann, Christine AU - Chijioke, Obinna AU - Quast, Isaak AU - Shilaih, Mohaned AU - Strouvelle, Victoria P AU - Neumann, Kathrin AU - Menter, Thomas AU - Dirnhofer, Stephan AU - Lam, Janice KP AU - Hui, Kwai F AU - Bredl, Simon AU - Schlaepfer, Erika AU - Sorce, Silvia AU - Zbinden, Andrea AU - Capaul, Riccarda AU - Lünemann, Jan D AU - Aguzzi, Adriano AU - Chiang, Alan KS AU - Kempf, Werner AU - Trkola, Alexandra AU - Metzner, Karin J AU - Manz, Markus G AU - Grundhoff, Adam AU - Speck, Roberto F AU - Münz, Christian TI - EBV renders B cells susceptible to HIV-1 in humanized mice AID - 10.26508/lsa.202000640 DP - 2020 Aug 01 TA - Life Science Alliance PG - e202000640 VI - 3 IP - 8 4099 - http://www.life-science-alliance.org/content/3/8/e202000640.short 4100 - http://www.life-science-alliance.org/content/3/8/e202000640.full SO - Life Sci. Alliance2020 Aug 01; 3 AB - HIV and EBV are human pathogens that cause a considerable burden to worldwide health. In combination, these viruses are linked to AIDS-associated lymphomas. We found that EBV, which transforms B cells, renders them susceptible to HIV-1 infection in a CXCR4 and CD4-dependent manner in vitro and that CXCR4-tropic HIV-1 integrates into the genome of these B cells with the same molecular profile as in autologous CD4+ T cells. In addition, we established a humanized mouse model to investigate the in vivo interactions of EBV and HIV-1 upon coinfection. The respective mice that reconstitute human immune system components upon transplantation with CD34+ human hematopoietic progenitor cells could recapitulate aspects of EBV and HIV immunobiology observed in dual-infected patients. Upon coinfection of humanized mice, EBV/HIV dual-infected B cells could be detected, but were susceptible to CD8+ T-cell–mediated immune control.