@article {McHughe202000640, author = {Donal McHugh and Renier Myburgh and Nicole Caduff and Michael Spohn and Yik Lim Kok and Christian W Keller and Anita Murer and Bithi Chatterjee and Julia R{\"u}hl and Christine Engelmann and Obinna Chijioke and Isaak Quast and Mohaned Shilaih and Victoria P Strouvelle and Kathrin Neumann and Thomas Menter and Stephan Dirnhofer and Janice KP Lam and Kwai F Hui and Simon Bredl and Erika Schlaepfer and Silvia Sorce and Andrea Zbinden and Riccarda Capaul and Jan D L{\"u}nemann and Adriano Aguzzi and Alan KS Chiang and Werner Kempf and Alexandra Trkola and Karin J Metzner and Markus G Manz and Adam Grundhoff and Roberto F Speck and Christian M{\"u}nz}, title = {EBV renders B cells susceptible to HIV-1 in humanized mice}, volume = {3}, number = {8}, elocation-id = {e202000640}, year = {2020}, doi = {10.26508/lsa.202000640}, publisher = {Life Science Alliance}, abstract = {HIV and EBV are human pathogens that cause a considerable burden to worldwide health. In combination, these viruses are linked to AIDS-associated lymphomas. We found that EBV, which transforms B cells, renders them susceptible to HIV-1 infection in a CXCR4 and CD4-dependent manner in vitro and that CXCR4-tropic HIV-1 integrates into the genome of these B cells with the same molecular profile as in autologous CD4+ T cells. In addition, we established a humanized mouse model to investigate the in vivo interactions of EBV and HIV-1 upon coinfection. The respective mice that reconstitute human immune system components upon transplantation with CD34+ human hematopoietic progenitor cells could recapitulate aspects of EBV and HIV immunobiology observed in dual-infected patients. Upon coinfection of humanized mice, EBV/HIV dual-infected B cells could be detected, but were susceptible to CD8+ T-cell{\textendash}mediated immune control.}, URL = {https://www.life-science-alliance.org/content/3/8/e202000640}, eprint = {https://www.life-science-alliance.org/content/3/8/e202000640.full.pdf}, journal = {Life Science Alliance} }