RT Journal Article
SR Electronic
T1 Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202000743
DO 10.26508/lsa.202000743
VO 3
IS 7
A1 Sandra E Joppé
A1 Loïc M Cochard
A1 Louis-Charles Levros, Jr
A1 Laura K Hamilton
A1 Pierre Ameslon
A1 Anne Aumont
A1 Fanie Barnabé-Heider
A1 Karl JL Fernandes
YR 2020
UL https://www.life-science-alliance.org/content/3/7/e202000743.abstract
AB The ventricular epithelium of the adult forebrain is a heterogeneous cell population that is a source of both quiescent and activated neural stem cells (qNSCs and aNSCs, respectively). We genetically targeted a subset of ventricle-contacting, glial fibrillary acidic protein (GFAP)-expressing cells, to study their involvement in qNSC/aNSC–mediated adult neurogenesis. Ventricle-contacting GFAP+ cells were lineage-traced beginning in early adulthood using adult brain electroporation and produced small numbers of olfactory bulb neuroblasts until at least 21 mo of age. Notably, electroporated GFAP+ neurogenic precursors were distinct from both qNSCs and aNSCs: they did not give rise to neurosphere-forming aNSCs in vivo or after extended passaging in vitro and they were not recruited during niche regeneration. GFAP+ cells with these properties included a FoxJ1+GFAP+ subset, as they were also present in an inducible FoxJ1 transgenic lineage-tracing model. Transiently overexpressing Mash1 increased the neurogenic output of electroporated GFAP+ cells in vivo, identifying them as a potentially recruitable population. We propose that the qNSC/aNSC lineage of the adult forebrain coexists with a distinct, minimally expanding subset of GFAP+ neurogenic precursors.