TY - JOUR T1 - Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000743 VL - 3 IS - 7 SP - e202000743 AU - Sandra E Joppé AU - Loïc M Cochard AU - Louis-Charles Levros, Jr AU - Laura K Hamilton AU - Pierre Ameslon AU - Anne Aumont AU - Fanie Barnabé-Heider AU - Karl JL Fernandes Y1 - 2020/07/01 UR - https://www.life-science-alliance.org/content/3/7/e202000743.abstract N2 - The ventricular epithelium of the adult forebrain is a heterogeneous cell population that is a source of both quiescent and activated neural stem cells (qNSCs and aNSCs, respectively). We genetically targeted a subset of ventricle-contacting, glial fibrillary acidic protein (GFAP)-expressing cells, to study their involvement in qNSC/aNSC–mediated adult neurogenesis. Ventricle-contacting GFAP+ cells were lineage-traced beginning in early adulthood using adult brain electroporation and produced small numbers of olfactory bulb neuroblasts until at least 21 mo of age. Notably, electroporated GFAP+ neurogenic precursors were distinct from both qNSCs and aNSCs: they did not give rise to neurosphere-forming aNSCs in vivo or after extended passaging in vitro and they were not recruited during niche regeneration. GFAP+ cells with these properties included a FoxJ1+GFAP+ subset, as they were also present in an inducible FoxJ1 transgenic lineage-tracing model. Transiently overexpressing Mash1 increased the neurogenic output of electroporated GFAP+ cells in vivo, identifying them as a potentially recruitable population. We propose that the qNSC/aNSC lineage of the adult forebrain coexists with a distinct, minimally expanding subset of GFAP+ neurogenic precursors. ER -