PT  - JOURNAL ARTICLE
AU  - Sandra E Joppé
AU  - Loïc M Cochard
AU  - Louis-Charles Levros, Jr
AU  - Laura K Hamilton
AU  - Pierre Ameslon
AU  - Anne Aumont
AU  - Fanie Barnabé-Heider
AU  - Karl JL Fernandes
TI  - Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium
AID  - 10.26508/lsa.202000743
DP  - 2020 Jul 01
TA  - Life Science Alliance
PG  - e202000743
VI  - 3
IP  - 7
4099  - https://www.life-science-alliance.org/content/3/7/e202000743.short
4100  - https://www.life-science-alliance.org/content/3/7/e202000743.full
SO  - Life Sci. Alliance2020 Jul 01; 3
AB  - The ventricular epithelium of the adult forebrain is a heterogeneous cell population that is a source of both quiescent and activated neural stem cells (qNSCs and aNSCs, respectively). We genetically targeted a subset of ventricle-contacting, glial fibrillary acidic protein (GFAP)-expressing cells, to study their involvement in qNSC/aNSC–mediated adult neurogenesis. Ventricle-contacting GFAP+ cells were lineage-traced beginning in early adulthood using adult brain electroporation and produced small numbers of olfactory bulb neuroblasts until at least 21 mo of age. Notably, electroporated GFAP+ neurogenic precursors were distinct from both qNSCs and aNSCs: they did not give rise to neurosphere-forming aNSCs in vivo or after extended passaging in vitro and they were not recruited during niche regeneration. GFAP+ cells with these properties included a FoxJ1+GFAP+ subset, as they were also present in an inducible FoxJ1 transgenic lineage-tracing model. Transiently overexpressing Mash1 increased the neurogenic output of electroporated GFAP+ cells in vivo, identifying them as a potentially recruitable population. We propose that the qNSC/aNSC lineage of the adult forebrain coexists with a distinct, minimally expanding subset of GFAP+ neurogenic precursors.