PT  - JOURNAL ARTICLE
AU  - Adam A Sivitilli
AU  - Jessica T Gosio
AU  - Bibaswan Ghoshal
AU  - Alesya Evstratova
AU  - Daniel Trcka
AU  - Parisa Ghiasi
AU  - J Javier Hernandez
AU  - Jean Martin Beaulieu
AU  - Jeffrey L Wrana
AU  - Liliana Attisano
TI  - Robust production of uniform human cerebral organoids from pluripotent stem cells
AID  - 10.26508/lsa.202000707
DP  - 2020 May 01
TA  - Life Science Alliance
PG  - e202000707
VI  - 3
IP  - 5
4099  - https://www.life-science-alliance.org/content/3/5/e202000707.short
4100  - https://www.life-science-alliance.org/content/3/5/e202000707.full
SO  - Life Sci. Alliance2020 May 01; 3
AB  - Human cerebral organoid (hCO) models offer the opportunity to understand fundamental processes underlying human-specific cortical development and pathophysiology in an experimentally tractable system. Although diverse methods to generate brain organoids have been developed, a major challenge has been the production of organoids with reproducible cell type heterogeneity and macroscopic morphology. Here, we have directly addressed this problem by establishing a robust production pipeline to generate morphologically consistent hCOs and achieve a success rate of >80%. These hCOs include both a radial glial stem cell compartment and electrophysiologically competent mature neurons. Moreover, we show using immunofluorescence microscopy and single-cell profiling that individual organoids display reproducible cell type compositions that are conserved upon extended culture. We expect that application of this method will provide new insights into brain development and disease processes.