TY - JOUR T1 - Genetic and molecular characterization of multicomponent resistance of <em>Pseudomonas</em> against allicin JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202000670 VL - 3 IS - 5 SP - e202000670 AU - Jan Borlinghaus AU - Anthony Bolger AU - Christina Schier AU - Alexander Vogel AU - Björn Usadel AU - Martin CH Gruhlke AU - Alan J Slusarenko Y1 - 2020/05/01 UR - https://www.life-science-alliance.org/content/3/5/e202000670.abstract N2 - The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant Pseudomonas fluorescens (PfAR-1), which was isolated from garlic, conferred allicin resistance to Pseudomonas syringae and even to Escherichia coli. Resistance-conferring genes had redox-related functions and were on core fragments from three similar genomic islands identified by sequencing and in silico analysis. Transposon mutagenesis and overexpression analyses revealed the contribution of individual candidate genes to allicin resistance. Taken together, our data define a multicomponent resistance mechanism against allicin in PfAR-1, achieved through horizontal gene transfer. ER -