TY - JOUR T1 - CSDE1 controls gene expression through the miRNA-mediated decay machinery JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201900632 VL - 3 IS - 4 SP - e201900632 AU - Pavan Kumar Kakumani AU - Louis-Mathieu Harvey AU - François Houle AU - Tanit Guitart AU - Fátima Gebauer AU - Martin J Simard Y1 - 2020/04/01 UR - https://www.life-science-alliance.org/content/3/4/e201900632.abstract N2 - In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-terminal domains. We show that CSDE1 interacts with LSM14A, a constituent of P-body assembly and further associates to the DCP1–DCP2 decapping complex, suggesting that CSDE1 could promote the decay of miRNA-induced silencing complex-targeted mRNAs. Together, our findings uncover a hitherto unknown mechanism used by CSDE1 in the control of gene expression mediated by the miRNA pathway. ER -