RT Journal Article
SR Electronic
T1 CSDE1 controls gene expression through the miRNA-mediated decay machinery
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201900632
DO 10.26508/lsa.201900632
VO 3
IS 4
A1 Pavan Kumar Kakumani
A1 Louis-Mathieu Harvey
A1 François Houle
A1 Tanit Guitart
A1 Fátima Gebauer
A1 Martin J Simard
YR 2020
UL https://www.life-science-alliance.org/content/3/4/e201900632.abstract
AB In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-terminal domains. We show that CSDE1 interacts with LSM14A, a constituent of P-body assembly and further associates to the DCP1–DCP2 decapping complex, suggesting that CSDE1 could promote the decay of miRNA-induced silencing complex-targeted mRNAs. Together, our findings uncover a hitherto unknown mechanism used by CSDE1 in the control of gene expression mediated by the miRNA pathway.