PT - JOURNAL ARTICLE AU - Pavan Kumar Kakumani AU - Louis-Mathieu Harvey AU - François Houle AU - Tanit Guitart AU - Fátima Gebauer AU - Martin J Simard TI - CSDE1 controls gene expression through the miRNA-mediated decay machinery AID - 10.26508/lsa.201900632 DP - 2020 Apr 01 TA - Life Science Alliance PG - e201900632 VI - 3 IP - 4 4099 - https://www.life-science-alliance.org/content/3/4/e201900632.short 4100 - https://www.life-science-alliance.org/content/3/4/e201900632.full SO - Life Sci. Alliance2020 Apr 01; 3 AB - In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-terminal domains. We show that CSDE1 interacts with LSM14A, a constituent of P-body assembly and further associates to the DCP1–DCP2 decapping complex, suggesting that CSDE1 could promote the decay of miRNA-induced silencing complex-targeted mRNAs. Together, our findings uncover a hitherto unknown mechanism used by CSDE1 in the control of gene expression mediated by the miRNA pathway.