RT Journal Article
SR Electronic
T1 Transglutaminase 2 mediates hypoxia-induced selective mRNA translation via polyamination of 4EBPs
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201900565
DO 10.26508/lsa.201900565
VO 3
IS 3
A1 Cho, Sung-Yup
A1 Lee, Seungun
A1 Yeom, Jeonghun
A1 Kim, Hyo-Jun
A1 Lee, Jin-Haeng
A1 Shin, Ji-Woong
A1 Kwon, Mee-ae
A1 Lee, Ki Baek
A1 Jeong, Eui Man
A1 Ahn, Hee Sung
A1 Shin, Dong-Myung
A1 Kim, Kyunggon
A1 Kim, In-Gyu
YR 2020
UL http://www.life-science-alliance.org/content/3/3/e201900565.abstract
AB Hypoxia selectively enhances mRNA translation despite suppressed mammalian target of rapamycin complex 1 activity, contributing to gene expression reprogramming that promotes metastasis and survival of cancer cells. Little is known about how this paradoxical control of translation occurs. Here, we report a new pathway that links hypoxia to selective mRNA translation. Transglutaminase 2 (TG2) is a hypoxia-inducible factor 1–inducible enzyme that alters the activity of substrate proteins by polyamination or crosslinking. Under hypoxic conditions, TG2 polyaminated eukaryotic translation initiation factor 4E (eIF4E)-bound eukaryotic translation initiation factor 4E-binding proteins (4EBPs) at conserved glutamine residues. 4EBP1 polyamination enhances binding affinity for Raptor, thereby increasing phosphorylation of 4EBP1 and cap-dependent translation. Proteomic analyses of newly synthesized proteins in hypoxic cells revealed that TG2 activity preferentially enhanced the translation of a subset of mRNA containing G/C-rich 5′UTRs but not upstream ORF or terminal oligopyrimidine motifs. These results indicate that TG2 is a critical regulator in hypoxia-induced selective mRNA translation and provide a promising molecular target for the treatment of cancers.