RT Journal Article
SR Electronic
T1 Gammaherpesvirus-infected germinal center cells express a distinct immunoglobulin repertoire
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201900526
DO 10.26508/lsa.201900526
VO 3
IS 3
A1 Monika A Zelazowska
A1 Qiwen Dong
A1 Joshua B Plummer
A1 Yi Zhong
A1 Bin Liu
A1 Laurie T Krug
A1 Kevin M McBride
YR 2020
UL https://www.life-science-alliance.org/content/3/3/e201900526.abstract
AB The gammaherpesviruses (γHVs), human Kaposi sarcoma-associated herpesvirus (KSHV), EBV, and murine γHV68 are prevalent infections associated with lymphocyte pathologies. After primary infection, EBV and γHV68 undergo latent expansion in germinal center (GC) B cells and persists in memory cells. The GC reaction evolves and selects antigen-specific B cells for memory development but whether γHV passively transients or manipulates this process in vivo is unknown. Using the γHV68 infection model, we analyzed the Ig repertoire of infected and uninfected GC cells from individual mice. We found that infected cells displayed the hallmarks of affinity maturation, hypermutation, and isotype switching but underwent clonal expansion. Strikingly, infected cells displayed distinct repertoire, not found in uninfected cells, with recurrent utilization of certain Ig heavy V segments including Ighv10-1. In a manner observed with KSHV, γHV68 infected cells also displayed lambda light chain bias. Thus, γHV68 subverts GC selection to expand in a specific B cell subset during the process that develops long-lived immunologic memory.