PT  - JOURNAL ARTICLE
AU  - Monika A Zelazowska
AU  - Qiwen Dong
AU  - Joshua B Plummer
AU  - Yi Zhong
AU  - Bin Liu
AU  - Laurie T Krug
AU  - Kevin M McBride
TI  - Gammaherpesvirus-infected germinal center cells express a distinct immunoglobulin repertoire
AID  - 10.26508/lsa.201900526
DP  - 2020 Mar 01
TA  - Life Science Alliance
PG  - e201900526
VI  - 3
IP  - 3
4099  - https://www.life-science-alliance.org/content/3/3/e201900526.short
4100  - https://www.life-science-alliance.org/content/3/3/e201900526.full
SO  - Life Sci. Alliance2020 Mar 01; 3
AB  - The gammaherpesviruses (γHVs), human Kaposi sarcoma-associated herpesvirus (KSHV), EBV, and murine γHV68 are prevalent infections associated with lymphocyte pathologies. After primary infection, EBV and γHV68 undergo latent expansion in germinal center (GC) B cells and persists in memory cells. The GC reaction evolves and selects antigen-specific B cells for memory development but whether γHV passively transients or manipulates this process in vivo is unknown. Using the γHV68 infection model, we analyzed the Ig repertoire of infected and uninfected GC cells from individual mice. We found that infected cells displayed the hallmarks of affinity maturation, hypermutation, and isotype switching but underwent clonal expansion. Strikingly, infected cells displayed distinct repertoire, not found in uninfected cells, with recurrent utilization of certain Ig heavy V segments including Ighv10-1. In a manner observed with KSHV, γHV68 infected cells also displayed lambda light chain bias. Thus, γHV68 subverts GC selection to expand in a specific B cell subset during the process that develops long-lived immunologic memory.