RT Journal Article
SR Electronic
T1 Laminin is the ECM niche for trophoblast stem cells
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201900515
DO 10.26508/lsa.201900515
VO 3
IS 2
A1 Daiji Kiyozumi
A1 Itsuko Nakano
A1 Ryoko Sato-Nishiuchi
A1 Satoshi Tanaka
A1 Kiyotoshi Sekiguchi
YR 2020
UL https://www.life-science-alliance.org/content/3/2/e201900515.abstract
AB The niche is a specialized microenvironment for tissue stem cells in vivo. It has long been emphasized that niche ECM molecules act on tissue stem cells to regulate their behavior, but the molecular entities of these interactions remain to be fully elucidated. Here, we report that laminin forms the in vivo ECM niche for trophoblast stem cells (TSCs), the tissue stem cells of the placenta. TSCs expressed fibronectin-binding, vitronectin-binding, and laminin-binding integrins, whereas the integrin ligands present in the TSC niche were collagen and laminin. Therefore, the only niche integrin ligand available for TSCs in vivo was laminin. Laminin promoted TSC adhesion and proliferation in vitro in an integrin binding–dependent manner. Importantly, when the integrin-binding ability of laminin was genetically ablated in mice, the size of the TSC population was significantly reduced compared with that in control mice. The present findings underscore an ECM niche function of laminin to support tissue stem cell maintenance in vivo.